Larvicidal potential of copper sulphide nano aqua dispersions against Aedes aegypti (Linnaeus)




Mosquito, metal nanoparticles, aqueous nanoformulations, vector control, mortality


Nanotechnology has emerged as promising field in insect pest management. Aedes aegypti (Linnaeus) a well-known vector of dengue, chikungunya, and dengue haemorrhagic fever has no commercial management practice for their eradication at the larval stage. In the present study, copper sulphide one of the most detoxified form of copper with biopotential properties was synthesised by standard methodology using sonochemical irradiation method and was evaluated for their larvicidal potential against Ae. aegypti. Treated larvae were observed for various morphological changes as compared to control. Larvae were most susceptible to CuSNPs at 7 ppm showing 100% mortality within 24 h. LC50 and LC90 values calculated with the help of POLO software were 4.42 and 5.73 ppm. The epithelium layer of treated larvae was damaged as compared to control. Remarkable results of copper sulphide nanoformulations at low dosage against Ae. aegypti larvae advocates their further exploration for vector control programmes.


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Atwa AA, Salah NA, Khafagi WE, Al-Ghamdi A. 2017. Insecticidal effects of pure and silver-doped copper oxide nanosheets on Spodoptera littoralis (Lepidoptera: noctuidae). Canadian Entomologist 149(5):677–690.

Baek SW, Kim MS. 2017. Antibacterial filter comprising copper based sulfur compound. US Patent 2016/0332104A1.

Baek SW. 2017. Artificial biomaterial comprising copper based compound. US Patent 2017/0035933A1.

Bar A, Andrew J. 2013. Morphology and morphometry of Aedes aegypti adult mosquito. Annual Research & Review in Biology 3:52–69.

Becker N, Petric D, Zgomba M, Boase C, Dahl C, Madon M, Kaiser A. 2010. Mosquitoes and their Control. 2nd ed. New York, U.S.A.: Springer Publications. p. 9–40.

Chakraborty P, Adhikar J, Chatterjee S, Biswas B, Chattopadhyay T. 2016. Facile synthesis of copper sulfide nanoparticles: antibacterial and antifungal activity study. Rasayan Journal of Chemistry. 9:77–83.

Deka B, Babu A, Baruah C, Barthakur M. 2021. Nanopesticides: A systematic review of their prospects with special reference to tea pest management. Frontiers in Nutrition. 8:686131.

Duhan JS, Kumar R, Kumar N, Kaur P, Nehra K, Duhan S. 2017. Nanotechnology: the new perspective in precision agriculture. Biotechnology Reports. 15:11–23.

Finney DJ. 1971. Probit Analysis. New York, U.S.A.: Cambridge University Press p. 68–72.

Gayathri B, Muthukumarasamy N, Velauthapillai D, Santhosh SB, Asokan V. 2018. Magnesium incorporated hydroxyapatite nanoparticles: preparation, characterisation, antibacterial and larvicidal activity. Arabian Journal of Chemistry 11(5):645–654.

Ghosh A, Chowdhury N, Chandra G. 2012. Plant extracts as potential mosquito larvicides. Indian Journal of Medical Research 135:581–598.

Goncalvez AP, Engle RE, St. Claire M, Purcell RH, Lai C-J. 2007. Monoclonal antibody-mediated enhancement of dengue virus infection in vitro and in vivo and strategies for prevention. Proceedings of National Academy of Sciences of USA 04:9422–9427.

Guo L, Panderi I, Yan DD, Szulak K, Li Y, Chen Y, Ma H, Niesen DB, Seeram N, Ahmed A, et al. 2013, A comparative study of hollow copper sulfide nanoparticles and hollow gold nanosphere on degradability and toxicity. ACS Nano 7(10):8780–8793.

Kaur N, Kocher DK, Sidhu A. 2019. Synthesis and testing of Eucalyptus globulosa oil-based nanoemulsion for its larvicidal potential against Aedes aegypti. African Entomology 27(2):433–438.

Kumar S, Arjun MC, Gupta SK, Nongkynrih B. 2018. Malaria, dengue and chikungunya in India – an update. Indian Journal of Medical Specialities 9:25–30.

Lee H, Song YJ, Kim BS. 2013.Biological synthesis of copper nanoparticles using Magnolia kobus leaf extract and their antibacterial activity. Journal of Chemical Technology and Biotechnology 88:1971–1977.

Magro M, Bramuzzo S, Baratella D, Ugolotti J, Zoppellaro G, Chemello G, Olivotto I, Ballarin C, Radaelli G, Arcaro B, et al. 2019. Self-assembly of chlorin-e6 on γ-Fe2O3 nanoparticles: application for larvicidal activity against Aedes aegypti. Journal of Photochemistry and Photobiology B: Biology 194:21–31.

Margulis-Goshen K, Magdassi S, Ishaaya I, Palli S, Horowitz A. Advanced Technologies for Managing Insect Pests. Dordrecht, The Netherlands: Springer; 2013. p. 295–314.

Mavundza EJ, Maharaj R, Chukwujekwu JC, Finnie JF, van Staden J. 2013. Larvicidal activity against Anopheles arabiensis of 10 South African plants that are traditionally used as mosquito repellents. South African Journal of Botany 88:86–89.

EMinal PS, Prakash S. 2019. Efficacy of bimetallic copper-zinc nanoparticles against larvae of microfilariae vector in laboratory. International Journal of Scientific Research 8:72–75.

Minal SP, Prakash S. 2018.Characterization and nano-efficacy study of palladium nanoparticles against larvae of Anopheles stephensi (Liston). International Journal of Advanced Engineering and Nanotechnology 3(10):1–5.

Mishra PM, Sahoo SK, Naik GK, Parida K. 2015. Biomimetic synthesis, characterization and mechanism of formation of stable silver nanoparticles using Averrhoa carambola L. leaf extract. Materials Letters 160:566–571.

Murugan K, Nataraj D, Madhiyazhagan P, Sujitha V, Chandramohan B, Panneerselvam C, Dinesh D, Chandirasekar R, Kovendan K, Suresh U, et al. 2016. Carbon and silver nanoparticles in the fight against the filariasis vector Culex quinquefasciatus: genotoxicity and impact on behavioral traits of non-target aquatic organisms. Parasitology Research 115(3):1071–1083.

Naik R, Prashantha SC, Nagabhushana H, Sharma SC, Nagabhushana BM, Nagaswarupa HP, Premkumar HB. 2014. Low temperature synthesis and photoluminescence properties of red emitting Mg2 SiO4: Eu3+nanophosphor for near UV light emitting diodes. Sensors and Actuators B Chemical 195:140–149.

Nwabor FO. 2019. Synthetic insecticides, phytochemicals and mosquito resistance. Academia Journal of Biotechnology 5:118–125.

Powell RJ, Gloria-Soria A, Kotsakiozi P. 2018. Recent history of Aedes aegypti: vector genomics and epidemiology records. Bioscience 68(11):854–860.

Prabhakar M, Tyagi BK, Chandrasekaran N, Mukherjee A. 2017. Biological nanopesticides: a greener approach towards the mosquito vector control. Environmental Science and Pollution Research 25:10151–10163.

Prabhavathi SP, Ranjith Ranjam SR, Mauthamuthu Johnson T. 2015. Microwave synthesis, characterization and biological activities of CuS and CdS nanoparticles. World Journal of Pharmaceutical Research 4:710–720.

Rai M, Ingle AP, Pandit R, Paralikar P, Shende S, Gupta I, Biswas JK, da Silva SS. 2018. Copper and copper nanoparticles: role in management of insect pests and pathogenic microbes. Nanotechnology Reviews 7(4):303–315.

Ramyadevi J, Jeyasubramanian K, Marikani A, Rajakumar G, Rahuman AA, Santhoshkumar T, Kirthi AV, Jayaseelan C, Marimuthu S. 2011. Copper nanoparticles synthesized by polyol process used to control hematophagous parasites. Parasitology Research 109(5):1403–1415.

Rao H, Sun W, Ye S, Yan W, Li Y, Peng H, Liu Z, Bian Z, Huang C. 2016. Solution-processed CuS NPs as an inorganic holeselective contact material for inverted planar perovskite solar cells. ACS Applied Materials & Interfaces 8(12):7800–7805.

Robertson JL, Russell RM, Savin NE. 1980. POLO: A User’s Guide to Probit or Logit Analysis. Berkeley, CA, U.S.A.: Pacific South-West Forest and Range Experiment Station.

Selvan SM, Anand VK, Govindaraju K, Tamilselvan S, Kumar VG, Subramanian KS, Kannan M, Raja K. 2018. Green synthesis of copper oxide nanoparticles and mosquito larvicidal activity against dengue, zika and chikungunya causing vector Aedes aegypti. IET Nanobiotechnology 12(8):1042–1046.

Shaker AM, Zaki AH, Rahim EFA, Khader MH. 2016. Novel CuO nanoparticles for pest management and pesticides degradation. Advances in Environment Biology 10:274–283.

Sidhu A, Barmota H, Bala A. 2017. Antifungal evaluation studies of copper sulfide aqua-nanoformulations and its impact on seed quality of rice (Oryza sativa). Applied Nanotechnology 7:681–689.

Soni N, Prakash S. 2012. Efficacy of fungus mediated silver and gold nanoparticles against Aedes aegypti larvae. Parasitology Research 110(1):175–184.

Suresh M, Jeevanandam J, Chan YS, Danquah MK, Kalaiarasi JMV. 2020. Opportunities for metal oxide nanoparticles as a potential mosquitocide. Bionanoscience 10(1):292–310.

Valodkar M, Jadeja RN, Thounaojam MC, Devkar RV, Thakore S. 2011. In vitro toxicity study of plant latex capped silver nanoparticles in human lung carcinoma cells. Materials Science and Engineering 31(8):1723–1728.

Vijayakumar S, Vinoj G, Malaikozhundan B, Shanthi S, Vaseeharan B. 2015. Plectranthus amboinicus leaf extract mediated synthesis of zinc oxide nanoparticles and its control of methicillin resistant Staphylococcus aureus biofilm and blood sucking mosquito larvae. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 137:886–891.

Wang Z, von dem Bussche A, Kabadi PK, Kane AB, Hurt RH. 2013. Biological and environmental transformation of copper-based nanomaterials. ACS Nano 7(10):8715–8727.

Wattanachai P, Tintanon B. 1999. Resistance of Aedes aegypti to chemical compounds in aerosol insecticide products in different areas of Bangkok, Thailand. Journal of Communicable Disease 25:188–191

WHO [World Health Organisation]. 2015. Dengue and severe dengue. Fact sheet No. 117. Geneva, Switzerland: World Health Organization

WHO [World Health Organisation] 2017.

WHO [World Health Organisation]. 2018. Biosafety and Biosecurity. Fact sheet 1. Geneva, Switzerland: World Health Organization.

Wilke BB, Marrelli MT. 2012. Genetic control of mosquitoes: population suppression strategies. Journal of the São Paulo Institute of Tropical Medicine 54(5):287–292.

Yadav S, Prakash S. 2016. Control of filariasis by silver nanoparticles: A green method for health care. In: Humanitarian Technology Conference (R10-HTC), IEEE Region 10 (pp. 1–7). IEEE.




How to Cite

Sandhu K, Vashishat N, Sidhu A. Larvicidal potential of copper sulphide nano aqua dispersions against Aedes aegypti (Linnaeus). Afr. Entomol. [Internet]. 2022 Aug. 16 [cited 2022 Oct. 3];30. Available from: