Assessment of antibacterial genes of emerging queen Apis mellifera L. induced by the bacterium Paenibacillus larvae larvae

Authors

  • SAS Gomaa Research and Training Centre on Vectors of Diseases, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
  • EMS Barakat Department of Entomology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
  • MS Salama Department of Entomology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
  • EE El Gohary Research and Training Centre on Vectors of Diseases https://orcid.org/0000-0001-9607-3092

DOI:

https://doi.org/10.17159/2254-8854/2022/a13586

Keywords:

Honey-bee queen, American foulbrood, antimicrobial peptides, abacien, defensin, RT-PCR

Abstract

This study evaluated the expression of antimicrobial abaecin and defensin genes in virgin honey-bee queens (Apis mellifera) following induction of 4th larval instar with the bacterium Paenibacillus larvae larvae (1.07 × 102 CFU/queen); to investigate whether the presence of bacteria affects the immune response as well as gene transcript levels for the immune proteins. The total body proteins of bacteria-treated queens showed a highly significant increase, and the appearance of new proteins patterns and/or disappearance of others. The gene expression profile of treated queens showed up-regulation equalled four-fold of the defensin gene, whereas no progression occurred in the abacien gene. This finding likewise greatly affects the diseases challenging this pollinator.

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Published

2022-09-19

How to Cite

1.
Gomaa S, Barakat E, Salama M, El Gohary E. Assessment of antibacterial genes of emerging queen Apis mellifera L. induced by the bacterium Paenibacillus larvae larvae. Afr. Entomol. [Internet]. 2022 Sep. 19 [cited 2022 Oct. 3];30. Available from: https://www.africanentomology.com/article/view/13586

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